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Regulated interaction of endothelin B receptor with caveolin-1 by Tomohiro Yamaguchi; Yasunobu Murata; Yoshinori Fujiyoshi; Tomoko Doi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Regulated interaction of endothelin B receptor with caveolin-1 about?

The peptide hormone endothelin transmits various signals through G protein‐coupled receptors, the endothelin type A (ET~A~R) and B (ET~B~R) receptors. Caveolae are specialized lipid rafts containing polymerized caveolins. We examined the interaction of ET~B~R with caveolin‐1, expressed in Sf9, COS‐1, and HEK293 cells, and its effects on the subcellular distribution and the signal transduction of ET~B~R. ET~B~R formed a complex with caveolin‐1 in cells in which these two proteins were coexpressed and in the mixture after purification and reconstitution (as examined by immunoprecipitation) suggesting the direct binding of ET~B~R with caveolin‐1. The complex formed efficiently only when the ET~B~R was ligand‐free or bound to an antagonist, RES‐701‐1, whereas the addition of ET‐1 or another antagonist, BQ788, dissociated the complex, suggesting the structural recognition of ET~B~R by caveolin‐1. In contrast, the ET~A~R bound to caveolin‐1 regardless of ligand binding. Caveolin‐1 utilized its scaffolding domain (residues 82–101) and the C‐terminal domain (residues 136–178) to bind to ET~B~R, as for other signalling molecules. Furthermore, the amount of ET~B~R localized in caveolae incre

Who reads Regulated interaction of endothelin B receptor with caveolin-1?

Author

Tomohiro Yamaguchi; Yasunobu Murata; Yoshinori Fujiyoshi; Tomoko Doi

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)