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Can I read Flow Injection Chemiluminescent Immunoassay with Para-phenylphenol and Sodium Tetraphenylborate as Synergistic Enhancers on EtoBox?
Flow Injection Chemiluminescent Immunoassay with Para-phenylphenol and Sodium Tetraphenylborate as Synergistic Enhancers by Jian-Xin Luo; Xiu-Cen Yang is a Chemistry article available to read on EtoBox.
What is Flow Injection Chemiluminescent Immunoassay with Para-phenylphenol and Sodium Tetraphenylborate as Synergistic Enhancers about?
A new approach of flow injection chemiluminescent immunoassay (FICLIA) with synergistic enhancement was developed. The synergistic action was significant in the chemiluminescence (CL) system of luminol-H 2 O 2 -HRP with two enhancers, para-phenylphenol (PPP) and sodium tetraphenylborate (NaTPB). The relative CL intensity was 128.84% stronger (compared to NaTPB alone) and 122.66% stronger (compared to PPP alone) using both enhancers than any one alone (both P < 0.001). While the present approach was applied to the determination of rabbit IgG as a model analyte, satisfactory results were obtained in the dynamic range of 2-60 g l -1 with a detection limit of 0.68 fmol per injection and a precision of 4.7-9.3%, and the recoveries were 92.5-99.4%. The results of rabbit IgG determination accorded with those of radio immunoassay (RIA). The lifetime of the immuno-column was considerably long: one column was applied to more than 200 determinations in 1 month without significant loss of activity.
Who reads Flow Injection Chemiluminescent Immunoassay with Para-phenylphenol and Sodium Tetraphenylborate as Synergistic Enhancers?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Jian-Xin Luo; Xiu-Cen Yang
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)