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Mechanisms Of α,β‐Methylene ATP‐Induced Inhibition In Rat Ileal Smooth Muscle: Involvement Of Intracellular Ca^2+^ Stores In Purinergic Inhibition by M Storr; H Franck; D Saur; V Schusdziarra; H‐D Allescher is a Medicine article available to read on EtoBox.

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## SUMMARY 1. In order to investigate purinergic effects on rat ileal smooth muscle, we used α,β‐methylene ATP (α,β‐MeATP), ATP, ADP and UTP. α,β‐Methylene ATP and ATP were the only agonists that caused a concentration‐dependent inhibition of carbachol‐precontracted smooth muscle. The inhibitory effect of α,β‐MeATP was completely blocked by pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulphonic acid (3 × 10^–5^ mol/L), a selective antagonist of the P2X > > P2Y receptor. 2. Using reverse transcription–polymerase chain reaction we demonstrated the presence of both, P2X and P2Y receptor mRNA within the rat ileal longitudinal muscle/myenteric plexus layer preparation. 3. The α,β‐MeATP‐induced inhibition was blocked in a concentration‐dependent manner in the presence of the K^+^ channel blocker apamin, but was unaffected by other K^+^ channel blockers, such as charybdotoxin (10^–7^ mol/L), 4‐aminopyridine (10^–4^ mol/L), glibenclamide (10^–5^ mol/L) and tetraethylammonium (10^–3^ mol/L). 4. The α,β‐MeATP‐induced inhibition was unaffected by pretreatment with atropine (10^–6^ mol/L), phentolamine (10^–6^ mol/L), propranolol (10^–6^ mol/L), nitrendipine (10^–7^ mol/L), pertussis toxin (10^–6^ m

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Author

M Storr; H Franck; D Saur; V Schusdziarra; H‐D Allescher

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0305-1870)

Published

2000

Language

EN

Field

Medicine (Health Sciences)