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Plasmatic and Cell-based Enhancement by Microparticles Originated from Platelets and Endothelial Cells Under Simulated in Vitro Conditions of a Dilutional Coagulopathy by Julia Boehm; Nadine Schäfer; Marc Maegele; Birgit Stümpges; Ursula Bauerfeind; Michael Caspers is a scholarly article available to read on EtoBox.

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## Abstract Background: Aggressive fluid management and other external factors may lead to hypothermia, acidosis and hemodilution (defined as Lethal Triad, LT) contributing to a trauma-induced coagulopathy (TIC) that worsens patients’ outcomes. Procoagulant microparticles (MP) are crucial players at the interface of cellular and plasmatic coagulation. However, their functions remain largely unexplored. This study aimed to characterize effects of MP subtypes and concentrations on functional coagulation under in vitro simulated conditions. Methods: Blood from eleven volunteers were collected to simulate in vitro conditions of hemodilution (HD) and LT, respectively. HD was induced by replacing a blood volume of 33% by crystalloids and for LT, samples were further processed by reducing the temperature to 32 °C and lowering the pH to 6.8. MP were obtained either from platelet concentrates (platelet-derived MP, PDMP) or from cell culture (ECV304 cells for endothelial-derived MP, EDMP) by targeted stimulation. After introducing MP to in vitro conditions, we measured their concentration-dependent effects (1.000, 10.000 and 15.000 MP/μl blood) on coagulation compared to whole blood (WB). Fo

Author

Julia Boehm; Nadine Schäfer; Marc Maegele; Birgit Stümpges; Ursula Bauerfeind; Michael Caspers

Publisher

Research Square Platform LLC

Published

2021

Language

EN