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Immunosensor‐based rapid quantitative detection of Newcastle disease virus antibodies using innovative gold immunochromatographic assay by F. Yang; Y. Li; X. Jin; Q. Xu; F. Cheng; X. Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Aims: A novel quantitative method for rapid newcastle disease virus (ndv) antibody detection was developed based on an innovative gold immunochromatographic assay with a quantitative immunosensor. ## Methods and results: Ndv antibody-detecting test strips containing a two-reaction system and double-test lines (t1, t2) were prepared. the test results were judged according to the signal ratio between the test and control lines as measured by the quantitative immunosensor. the minimum detection limit of the test strips for ndv antibodies was 2 titres. in addition, the assay was highly specific because only ndv antibodies produced visible test lines on the strip. the clinical application of the strips was tested by detecting ndv antibodies in 506 serum samples collected from chickens. the results showed a coincidence of 92·49% with those of the haemagglutination inhibition assay. ## Conclusions: The strips were successfully prepared and showed high specificity towards ndv, sensitivity and stability. ## Significance and impact of the study: This study describes a new method for detection of ndv antibody and provides a reference basis for rapid and quantitative monitoring of ndv antib

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Author

F. Yang; Y. Li; X. Jin; Q. Xu; F. Cheng; X. Wang

Publisher

Oxford University Press (OUP)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)