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This study presents two real-time quantitative PCR (QPCR) methods for determining the plasmid copy number of pBR322 in recombinant Escherichia coli DH5α cultures. Both absolute and relative quantification methods were tested, yielding highly reproducible results that corresponded to previously reported values of 15-20 plasmid copies per cell. The methods are simpler and more cost-effective than traditional techniques, making them suitable for routine analysis of plasmid stability in recombinant systems.

Author

Nathan Simanjuntak

Language

EN