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Can I read Southern-blot analysis and simultaneous In Situ detection of hepatitis B virus–associated DNA and antigens in patients with end-stage liver disease on EtoBox?
Southern-blot analysis and simultaneous In Situ detection of hepatitis B virus–associated DNA and antigens in patients with end-stage liver disease by Kwang Hyub Han; F. Blaine Hollinger; Christine A. Noonan; Harvey Solomon; Goran B. G. Klintmalm; Robert M. Genta; Boris Yoffe is a Medicine article available to read on EtoBox.
What is Southern-blot analysis and simultaneous In Situ detection of hepatitis B virus–associated DNA and antigens in patients with end-stage liver disease about?
To gain new insights into the pathogenesis of hepatitis B virus-induced chronic liver disease, we have used nonisotopic in situ detection methods for the simultaneous analysis of hepatitis B virus DNA and antigens at the single-cell level. Paraffin-embedded liver specimens from 23 cirrhotic patients (12 HBsAg positive and 11 HBsAg negative) who underwent liver transplantation were evaluated by in situ hybridization with a digoxigenin-labeled DNA probe and digoxigenin detection system and by immunohistochemistry with an enhanced biotin-streptavidin technique. DNAs extracted from liver and serum specimens were analyzed by Southern-and slot-blot hybridization, respectively. Using the in situ techniques, we detected hepatitis B virus-specific DNA and antigens in 11 of 12 HBsAg-positive patients and in none of the 11 HBsAg-negative individuals. Replicative intermediates of hepatitis B virus DNA were detected by Southern-blot analysis in the same 11 HBsAg-positive patients, 6 of whom had no serological markers of hepatitis B virus replication. Therefore a good correlation was found between the results obtained by the in situ and Southern-blot hybridization analyses of tissue specimens. H
Who reads Southern-blot analysis and simultaneous In Situ detection of hepatitis B virus–associated DNA and antigens in patients with end-stage liver disease?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Kwang Hyub Han; F. Blaine Hollinger; Christine A. Noonan; Harvey Solomon; Goran B. G. Klintmalm; Robert M. Genta; Boris Yoffe
- Publisher
- Ovid Technologies (Wolters Kluwer Health)
- Published
- 1993
- Language
- EN
- Field
- Medicine (Health Sciences)