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Antibiotic susceptibility and genotypic characterization of Haemophilus influenzae strains isolated from nasopharyngeal specimens from children in day-care centers in eastern France by D. Talon; J. Leroy; M. J. Dupont; X. Bertrand; F. Mermet; M. Thouverez; J. M. Estavoyer is a Medicine article available to read on EtoBox.

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## Objective: To determine the overall carriage rate for haemophilus influenzae in young children in day-care centers, the frequency of resistance to various classes of antibiotic, and the clonal relationship between isolates of the various resistant phenotypes. ## Methods: Nasopharyngeal (np) specimens were obtained and cultured on chocolate agar with bacitracin. antibiotic susceptibility testing and serotyping were performed for all isolates. the genetic polymorphism of ampicillin-susceptible and beta-lactamase-producing isolates was studied by pulsed-field gel electrophoresis using smai. ## Results: Of the 596 np secretion cultures, 152 (25.5%) were positive for h. influenzae. sixty-four (42.1%) isolates produced beta-lactamase and two (1.3%) were ampicillin resistant but did not produce beta-lactamase. we were unable to serotype 150 isolates; one isolate belonged to capsular serotype e and one to serotype f. forty-six major dna patterns were identified among 76 randomized isolates. beta-lactamase producing isolates more frequently showed ep than ampicillin-susceptible isolates p < 10(-4). the frequency of isolates with ep was significantly lower in day-care centers attended by

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Author

D. Talon; J. Leroy; M. J. Dupont; X. Bertrand; F. Mermet; M. Thouverez; J. M. Estavoyer

Publisher

John Wiley and Sons; Elsevier ; Elsevier Limited; Elsevier BV; Test accounts (ISSN 1198-743X)

Published

2000

Language

EN

Field

Medicine (Health Sciences)