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Obtention of a Human Primary Humoral Response Against Schistosome Protective Antigens in Severe Combined Immunodeficiency Mice After the Transfer of Human Peripheral Blood Mononuclear Cells by Christine Mazingue; Françoise Cottrez; Claude Auriault; Jean-Yves Cesbron; André Capron is a Immunology and Microbiology article available to read on EtoBox.
What is Obtention of a Human Primary Humoral Response Against Schistosome Protective Antigens in Severe Combined Immunodeficiency Mice After the Transfer of Human Peripheral Blood Mononuclear Cells about?
Obtention of a human primary humoral response against schistosome protective antigens in severe combined immunodeficiency mice after the transfer of human peripheral blood mononuclear cells Peripheral blood mononuclear cells (PBMC) from healthy donors were injected into C.B.-17 severe combined immunodeficiency (scid) mice which were subsequently immunized with crude Schistosoma mansoni adult worm antigenic preparation (SWAP) or with recombinant S. mansoni 28-kDa glutathione transferase (r-Sm-28-GST) antigen. PBMC from a S. mansoni-infected patient were also transfered. The specific human anti-SWAP and anti-Sm-28-GST antibody responses were monitored.The presence in both cases of human specific antibodies in scid mouse sera was determined by enzyme-linked immunosorbent assay and Western blotting techniques using anti-human immunoglobulin reagents. No antibodies were detected in these sera using anti-mouse immunoglobulin antisera. These antibodies were functional since a cytotoxic activity against schistosomula was observed when monocytes were incubated with scid mouse sera positive for anti-Sm-28-GST antibodies.
Who reads Obtention of a Human Primary Humoral Response Against Schistosome Protective Antigens in Severe Combined Immunodeficiency Mice After the Transfer of Human Peripheral Blood Mononuclear Cells?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Christine Mazingue; Françoise Cottrez; Claude Auriault; Jean-Yves Cesbron; André Capron
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0014-2980)
- Published
- 1991
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)