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CD154 stimulation of interleukin-12 synthesis in human endothelial cells by Bianca Lienenlüke; Tieno Germann; Richard A. Kroczek; Markus Hecker is a Immunology and Microbiology article available to read on EtoBox.

What is CD154 stimulation of interleukin-12 synthesis in human endothelial cells about?

The interaction of proinflammatory type 1 T helper (Th1) cells expressing the CD40 ligand (CD154) with endothelial cells expressing the corresponding receptor (CD40) may play an important role in chronic inflammation including arteriosclerosis. Here we demonstrate that activation of CD40 in human cultured endothelial cells (e.g. by interaction with freshly isolated human T cells) not only up-regulates expression of various adhesion molecules, chemokines and cytokines, but within 12-24 h also causes the release of bioactive interleukin-12 (IL-12 p70) through induction of IL-12 p40 synthesis. IL-12 p35, on the other hand, appears to be constitutively expressed in these cells. Despite enhancing expression of the other gene products, cytokines such as interferon-gamma (IFN-gamma) or tumor necrosis factor-alpha, alone or in combination, failed to induce IL-12 p40 expression, whereas IFN-gamma markedly augmented CD154-induced IL-12 p40 and p70 release. Of note was that the magnitude of CD154-induced IL-12 synthesis in the cultured endothelial cells was comparable to that evoked in freshly isolated human monocytes. This CD40-mediated induction of endothelial IL-12 synthesis may thus lead

Who reads CD154 stimulation of interleukin-12 synthesis in human endothelial cells?

Author

Bianca Lienenlüke; Tieno Germann; Richard A. Kroczek; Markus Hecker

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0014-2980)

Published

2000

Language

EN

Field

Immunology and Microbiology (Life Sciences)