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A Simple Method of Preparing Diverse Neoagaro-oligosaccharides with β-Agarase by Jingbao Li; Feng Han; Xinzhi Lu; Xiaoyan Fu; Cuiping Ma; Yan Chu; Wengong Yu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Simple Method of Preparing Diverse Neoagaro-oligosaccharides with β-Agarase about?

In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant b-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and 13 C NMR spectroscopy.

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Author

Jingbao Li; Feng Han; Xinzhi Lu; Xiaoyan Fu; Cuiping Ma; Yan Chu; Wengong Yu

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)