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Quantification of Trandolapril and Its Metabolite Trandolaprilat in Human Plasma by Liquid Chromatography/tandem Mass Spectrometry Using Solid-phase Extraction by Ramakrishna V. S. Nirogi; Vishwottam N. Kandikere; Wishu Shrivastava; Koteshwara Mudigonda is a Chemistry article available to read on EtoBox.
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## Abstract A sensitive high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (MS/MS) method was developed and validated for the simultaneous quantification of trandolapril and its metabolite trandolaprilat in human plasma using ramipril as an internal standard. Following solid‐phase extraction, the analytes were separated using an isocratic mobile phase on a reversed‐phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M–H]^−^ ions, __m/z__ 429/168 for trandolapril, __m/z__ 401/168 for trandolaprilat and __m/z__ 415/166 for the internal standard. The method exhibited a linear dynamic range of 20–10 000 pg/mL for both trandolapril and trandolaprilat in human plasma. The lower limit of quantification was 20 pg/mL for both trandolapril and its metabolite. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence
Who reads Quantification of Trandolapril and Its Metabolite Trandolaprilat in Human Plasma by Liquid Chromatography/tandem Mass Spectrometry Using Solid-phase Extraction?
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- Author
- Ramakrishna V. S. Nirogi; Vishwottam N. Kandikere; Wishu Shrivastava; Koteshwara Mudigonda
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2006
- Language
- EN
- Field
- Chemistry (Physical Sciences)