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Characterization of the hypo-osmolarity-induced Ca2+ response in cultured rat astrocytes by Richard Fischer; Freimut Schliess; Dieter Hussinger is a Neuroscience article available to read on EtoBox.

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The influence of astrocyte swelling on the cytosolic free calcium concentration [Ca 21 ] i was studied at the single cell level. Sudden exposure of normo-osmotically (305 mosmol/l) cultured astrocytes to hypo-osmotic medium induced a biphasic increase in cytosolic calcium with an initial peak followed by a sustained plateau. The response was osmolarity dependent and was maximal at 205 mosmol/l with respect to [Ca 21 ] i and the percentage of responding cells. Other modes of astrocyte swelling [gradual adjustment of hypo-osmolarity, normo-osmotic exposure of hyper-osmotic (405 mosmol/l) maintained cells] produced a much weaker [Ca 21 ] i response. Change from 405 to 205 mosmol/l, however, resulted in the entire peak and an increased plateau. Experiments with Ca 21 -free medium and after pretreatment with BAPTA-AM, thapsigargin, phorbol myristate acetate, or nimodipine revealed that the peak mainly resulted from depletion of intracellular Ca 21 stores, whereas the plateau was probably due to capacitative Ca 21 entry and Ca 21 influx independent of store depletion including a nimodipin-sensitive component. Prior depletion of ryanodine-, bradykinin-or ATP-sensitive stores revealed that

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Author

Richard Fischer; Freimut Schliess; Dieter Hussinger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

1997

Language

EN

Field

Neuroscience (Life Sciences)