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This document discusses the development of a novel radiolabeling technique for bacterial outer membrane vesicles (OMVs) using positron emission tomography (PET) to enhance imaging and biodistribution studies. A genetically engineered E. coli strain was created to produce OMVs efficiently, and the SpyCatcher-SpyTag system was utilized for stable and specific radiolabeling. The method demonstrated high radiochemical purity and efficiency, paving the way for potential applications in vaccine and drug delivery

Author

Ramiro Caso

Language

EN