About this document
Gbmas by shxxx is a document available to read on EtoBox.
The document outlines a protocol for Genotyping by Multiplexing Amplicon Sequencing (GBMAS), detailing steps for primer preparation, sample aliquoting, PCR setup, and amplicon pooling. It includes specific instructions for preparing PCR mixes, conducting touchdown PCR cycles, and purifying the resulting DNA using AMPure beads. The final step involves quantifying the purified PCR pool for next-generation sequencing (NGS) library construction.
- Author
- shxxx
- Language
- EN