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Direct Isolation of Circulating Extracellular Vesicles from Blood for Vascular Risk Profiling in Type 2 Diabetes Mellitus by Hui Min Tay; Sheng Yuan Leong; Xiaohan Xu; Fang Kong; Megha Upadya; Rinkoo Dalan; Chor Yong Tay; Ming Dao; Subra Suresh; Han Wei Hou is a Engineering article available to read on EtoBox.
What is Direct Isolation of Circulating Extracellular Vesicles from Blood for Vascular Risk Profiling in Type 2 Diabetes Mellitus about?
Extracellular vesicles (EVs) are key mediators of communication among cells, and clinical utilities of EVs-based biomarkers remain limited due to difficulties in isolating EVs from whole blood reliably. We report a novel inertial-based microfluidic platform for direct isolation of nanoscale EVs (exosomes, 50 to 200 nm) and medium-sized EVs (microvesicles, 200 nm to 1 μm) from blood with high efficiency (three-fold increase in EV yield compared to ultracentrifugation). In a pilot clinical study of healthy (n = 5) and type 2 diabetes mellitus (T2DM, n = 9) subjects, we detected higher EV levels in T2DM patients (P < 0.05), and identified a subset of "high-risk" T2DM subjects with abnormally high (∼10-fold to 50-fold) amounts of platelet (CD41a+) or leukocyte-derived (CD45+) EVs. Our in vitro endothelial cell assay further revealed that EVs from "high-risk" T2DM subjects induced significantly higher vascular inflammation (ICAM-1 expression) (P < 0.05) as compared to healthy and non-"high-risk" T2DM subjects, reflecting a pro-inflammatory phenotype. Overall, the EV isolation tool is scalable, and requires less manual labour, cost and processing time. This enables further development of
Who reads Direct Isolation of Circulating Extracellular Vesicles from Blood for Vascular Risk Profiling in Type 2 Diabetes Mellitus?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Hui Min Tay; Sheng Yuan Leong; Xiaohan Xu; Fang Kong; Megha Upadya; Rinkoo Dalan; Chor Yong Tay; Ming Dao; Subra Suresh; Han Wei Hou
- Published
- 2021
- Language
- EN
- Field
- Engineering (Physical Sciences)