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Role of PPAR-γ in diabetes-induced testicular dysfunction, oxidative DNA damage and repair in leptin receptor-deficient obe ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Animals 2 2.2 Experimental protocol 2 2.3 Tissue collection and processing 2 2.4 Sperm count and sperm motility analysis 2 2.5 Histopathological analysis of the testis 2 2.6 8-oxo-dG dot blotting 2 2.7 8-oxo-dg immunohistochemistry 2 2.8 Western blotting for 4-HNE and BER pathway proteins 3 2.9 TUNEL assay 3 2.10 Statistical analysis 3 3 Results 3 3.1 The PPAR-γ stimulation increases bodyweights and reduces hyperglycemia in diabetic mice 3 3.2 Effects of PPAR-γ modulation on reproductive organs, sperm count, and sperm motility in mice 3 3.3 Effects of PPAR-γ modulation on testicular structure in mice 4 3.4 Effects of PPAR-γ modulation on diabetes-induced oxidative stress and DNA damage in testis 4 3.5 Effects of PPAR-γ modulation on proteins of base-excision repair pathway in the testis 4 4 Discussion 5 Author contributions 8 Declaration of competing interest 8 Acknowledgment 8 References 9

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nonfiction