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Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays by Varda Shkap; I Cohen; B Leibovitz; Savitsky; E Pipano; G Avni; S Shofer; U Giger; L Kappmeyer; D Knowles is a Immunology and Microbiology article available to read on EtoBox.

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Sera from 361 horses were tested by indirect immunofluorescence antibody test (IFA) and by competitive inhibition ELISA (cELISA), to detect antibodies to Babesia equi. The concordance between the assays was 95.7%. Application of a cutoff based on a calculated percent inhibition of 20% inhibition was used. Approximately one-third of all the horses tested were found serologically positive to B. equi, with more horses testing positive from northern Israel. Among horses raised with access to pasture there was a significant difference in the percentage of seropositive reactors (76.6% in the north and 20.1% in the central region), compared with horses without access to pasture (14.3 and 10.3%, respectively). Nineteen percent of stallions were found to be positive, which was significantly less than the proportions of seropositive mares and geldings: 38 and 42%, respectively. No significant association was found between the mean age of horses and seroreactivity to B. equi.

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Author

Varda Shkap; I Cohen; B Leibovitz; Savitsky; E Pipano; G Avni; S Shofer; U Giger; L Kappmeyer; D Knowles

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)

Published

1998

Language

EN

Field

Immunology and Microbiology (Life Sciences)