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Optimisation Technique for Stepwise Gradient Elution in Reversed-phase Liquid Chromatography by P Nikitas; A Pappa-Louisi; K Papachristos is a Chemistry article available to read on EtoBox.

What is Optimisation Technique for Stepwise Gradient Elution in Reversed-phase Liquid Chromatography about?

An optimisation technique of reversed-phase liquid chromatographic separations based on gradient elution with a stepwise variation pattern of the volume fraction φ of the organic modifier in the water-organic mobile phase is presented. It uses a non-linear least-squares programme with a Monte-Carlo search for initial estimates in order to determine the best variation pattern that leads to the optimum separation of a mixture of solutes. The validity of the above methodology was tested by separating eight catechol-related solutes with mobile phases modified by methanol or acetonitrile and variation patterns of two, three or four steps in the φ values. It was found in all cases a very satisfactory accuracy of the predicted gradient elution times, which is of the same order with the accuracy of the retention times predicted under isocratic or linear gradient conditions. In addition, it was shown that the proposed optimisation technique is both effective and flexible but well-shaped chromatograms are obtained under electrochemical detection only if steps with increasing φ are used and the change in φ is programmed to occur at the intermediate of the predicted peaks.

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Author

P Nikitas; A Pappa-Louisi; K Papachristos

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2004

Language

EN

Field

Chemistry (Physical Sciences)