Opening book details…
Can I read Inhibition of cell growth by EGR-1 in human primary cultures from malignant glioma on EtoBox?
Inhibition of cell growth by EGR-1 in human primary cultures from malignant glioma by Antonella Calogero; Vincenza Lombari; Giorgia De Gregorio; Antonio Porcellini; Severine Ucci; Antonietta Arcella; Riccardo Caruso; Franco Maria Gagliardi; Alberto Gulino; Gaetano Lanzetta; Luigi Frati; Dan Mercola; Giuseppe Ragona is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Inhibition of cell growth by EGR-1 in human primary cultures from malignant glioma about?
## Background: The aim of this work was to investigate in vitro the putative role of EGR-1 in the growth of glioma cells. EGR-1 expression was examined during the early passages in vitro of 17 primary cell lines grown from 3 grade III and from 14 grade IV malignant astrocytoma explants. The explanted tumors were genetically characterized at the p53, MDM2 and INK4a/ARF loci, and fibronectin expression and growth characteristics were examined. A recombinant adenovirus overexpressing EGR-1 was tested in the primary cell lines. Results: Low levels of EGR-1 protein were found in all primary cultures examined, with lower values present in grade IV tumors and in cultures carrying wild-type copies of p53 gene. The levels of EGR-1 protein were significantly correlated to the amount of intracellular fibronectin, but only in tumors carrying wild-type copies of the p53 gene (R = 0,78, p = 0.0082). Duplication time, plating efficiency, colony formation in agarose, and contact inhibition were also altered in the p53 mutated tumor cultures compared to those carrying wild-type p53. Growth arrest was achieved in both types of tumor within 1-2 weeks following infection with a recombinant adenovirus
Who reads Inhibition of cell growth by EGR-1 in human primary cultures from malignant glioma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Antonella Calogero; Vincenza Lombari; Giorgia De Gregorio; Antonio Porcellini; Severine Ucci; Antonietta Arcella; Riccardo Caruso; Franco Maria Gagliardi; Alberto Gulino; Gaetano Lanzetta; Luigi Frati; Dan Mercola; Giuseppe Ragona
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1475-2867)
- Published
- 2004
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)