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Can I read Permeabilization of Escherichia coli cells in the biotransformation of trimethylammonium compounds into l-carnitine on EtoBox?
Permeabilization of Escherichia coli cells in the biotransformation of trimethylammonium compounds into l-carnitine by M. Cánovas; T. Torroglosa; J.L. Iborra is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Permeabilization of Escherichia coli cells in the biotransformation of trimethylammonium compounds into l-carnitine about?
The activity of permeabilized cells of Escherichia coli strains during the biotransformation of trimethylamonium compounds, such as crotonobetaine and d-carnitine, into l-carnitine was determined. Permeabilization of cells did not affect the biotransformation of d-carnitine but improved the yield of l-carnitine obtained when crotonobetaine was used as biotransformation substrate (by nearly two-fold). A higher degree of cell activity with respect to control (45% yield) was observed with organic solvents such as acetone, ethanol, isopropyl alcohol and toluene (65-70% yield), detergents such as Triton X-100 and Tween 20 (70-75% yield) and polyethylenimine (80-89% yield). However, maximum cell activity was attained with 2% of the detergents, Triton X-100 and Tween 20, and 0.1% of polyethylenimine, using a temperature of 37 • C and 10-30 min of treatment time, in which case a yield of 75-89% (100% increase) was obtained with E. coli O44 K74 and 85-94% (80% increase) with E. coli K38 T7-5KE32. Both detergents and polyethylenimine produced alterations in the outer membrane of treated cells since thin-sectioned bacteria were observed by transmision electron microscopy. No l-carnitine dehyd
Who reads Permeabilization of Escherichia coli cells in the biotransformation of trimethylammonium compounds into l-carnitine?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- M. Cánovas; T. Torroglosa; J.L. Iborra
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)