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Involvement of protein kinase and G proteins in the signal transduction of benzophenanthridine alkaloid biosynthesis by Gail B. Mahady; Chuan Liu; Chris W.W. Beecher is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We have previously reported that elicitor-induced benzophenanthridine alkaloid biosynthesis in suspension-cell cultures of Sanguinaria canadensis L. (SCP-GM) is mediated by a signal transduction system that involves calcium and possibly protein kinase(s). In this work, a number of exogenous agents were employed to further investigate the components of the signal transduction pathway involved in the induction of alkaloid biosynthesis by a fungal elicitor and abscisic acid (ABA). SCP-GM suspension-cells were treated with compounds that modify protein kinase activity, including phorbol esters, and 1-oleoyl-2-acetyl-rac-glycerol (OAG), a synthetic diacylglycerol analogue. Phorbol-12-myristate-13-acetate induced alkaloid accumulation by as much as 65-fold over control values, while the negative control, phorbol-13-monoacetate, had no effect. OAG also increased alkaloid production by approximately 25-fold as compared to controls. Likewise, pretreatment of the suspension-cell cultures with H-7 or staurosporine, significantly suppressed ABA-or fungal-induction of benzophenanthridine alkaloid biosynthesis. Modulators of GTP-binding protein activity were also active in this system. Treatment

Who reads Involvement of protein kinase and G proteins in the signal transduction of benzophenanthridine alkaloid biosynthesis?

Author

Gail B. Mahady; Chuan Liu; Chris W.W. Beecher

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)