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Chromosome site‐specific immunohistochemical detection of DNA adducts in N‐acetoxy‐2‐acetylaminofluorene‐exposed chinese hamster ovary cells by Ofelia A. Olivero; Henrik Huitfeldt; Miriam C. Poirier is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Chromosome site‐specific immunohistochemical detection of DNA adducts in N‐acetoxy‐2‐acetylaminofluorene‐exposed chinese hamster ovary cells about?
## Abstract In these studies a polyclonal antiserum elicited against a carcinogen‐DNA adduct was used to explore the localization of DNA adducts in metaphase chromosomes of cultured cells. Morphological visualization of the adduct __N__‐(deoxyguanosin‐8‐yl)‐2‐aminofluorene (dG‐C8‐AF) in Chinese hamster ovary (CHO) cells exposed to the direct‐acting carcinogen __N__‐acetoxy‐2‐acetylaminofluorene (N‐Ac‐AAF) was accomplished by indirect immunofluorescence with an anti‐G‐C8‐AF antiserum. At the same time the pattern of chromosomal DNA replication was determined by replicative incorporation of bromodeoxyuridine (BrdUrd) and chromosomal staining with anti‐BrdUrd. Visualization of DNA in chromosomes was accomplished with Hoechst 33258 dye. When synchronized CHO cells were exposed to __N__‐Ac‐AAF for 0.5 h during early S phase, the chromosomal pattern of dG‐C8‐AF adduct formation was not random. Metaphase chromosome spreads from cells exposed to __N__‐Ac‐AAF in different experiments contained certain chromosome regions that had a consistently high adduct concentration. The regions of high DNA damage corresponded to the regions active in DNA synthesis when BrdUrd and the carcinogen were giv
Who reads Chromosome site‐specific immunohistochemical detection of DNA adducts in N‐acetoxy‐2‐acetylaminofluorene‐exposed chinese hamster ovary cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ofelia A. Olivero; Henrik Huitfeldt; Miriam C. Poirier
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)
- Published
- 1990
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)