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Electrotaxis-mediated cell motility and nutrient availability determine Chlamydomonas microsphaera-surface interactions in bioelectrochemical systems 1 1 Introduction 1 2 Materials and methods 2 2.1 Strains, medium and growth condition 2 2.2 Bioreactor assembly and configuration 2 2.3 Nutrient adsorption at various initial concentrations and applied voltages 3 2.4 C. microsphaera attachment at various initial nutrient concentrations and applied voltages 3 2.5 Measurement of microalgal cell motility 3 2.6 Staining and confocal laser scanning microscopy observations 3 2.7 EPS, zeta potential and chemical analysis 3 2.8 Statistical analysis 3 3 Results and discussion 4 3.1 C. microsphaera attachment under various applied voltages 4 3.2 Nutrient adsorption under various applied voltages 4 3.3 External voltage loading alters cell motility patterns 5 3.4 Dominant factors driving C. microsphaera attachment with various applied voltages under various nutrient concentrations 6 4 Discussion 6 5 Conclusions 7 Declaration of Competing Interest 8 Acknowledgments 8 Appendix A Supplementary data 8 References 8

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Language

EN

Category

nonfiction