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Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate by Luis Manuel Rosales-Colunga; Elías Razo-Flores; Leandro G. Ordoñez; Felipe Alatriste-Mondragón; Antonio De León-Rodríguez is a Engineering article available to read on EtoBox.
What is Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate about?
This study reports a fermentative hydrogen production by Escherichia coli using cheese whey as substrate. To improve the biohydrogen production, an E. coli DhycA DlacI strain (WDHL) was constructed. The absence of hycA and lacI genes had a positive effect on the biohydrogen production. The strain produced 22% more biohydrogen in a shorter time than the wild-type (WT) strain. A Box-Behnken experimental design was used to optimize pH, temperature and substrate concentration. The optimal initial conditions for biohydrogen production by WDHL strain were pH 7.5, 37 C and 20 g/L of cheese whey. The specific production rate was improved from 3.29 mL H 2 /optical density at 600 nm (OD 600nm ) unit-h produced by WDHL under non-optimal conditions to 5.88 mL H 2 /OD 600nm unit-h under optimal conditions. Using optimal initial conditions, galactose can be metabolized by WDHL strain. The maximum yield obtained was 2.74 mol H 2 /mol lactose consumed, which is comparable with the yield reached in other hydrogen production processes with Clostridium sp. or mixed cultures.
Who reads Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Luis Manuel Rosales-Colunga; Elías Razo-Flores; Leandro G. Ordoñez; Felipe Alatriste-Mondragón; Antonio De León-Rodríguez
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0360-3199)
- Published
- 2010
- Language
- EN
- Field
- Engineering (Physical Sciences)