About this Biochemistry, Genetics and Molecular Biology article

Regulation of RssB‐dependent proteolysis in Escherichia coli : a role for acetyl phosphate in a response regulator‐controlled process by Sandrine Bouché; Eberhard Klauck; Daniela Fischer; Magnus Lucassen; Kirsten Jung; Regine Hengge‐Aronis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

Sigma(S) (RpoS) is a highly unstable global regulatory protein in Escherichia coli, whose degradation is inhibited by various stress signals, such as carbon starvation, high osmolarity and heat shock. As a consequence, these stresses result in the induction of sigma(S)-regulated stress-protective proteins. The two-component-type response regulator, RssB, is essential for the rapid proteolysis of sigma(S) and is probably involved in the transduction of some of these stress signals. Acetyl phosphate can be used as a phosphodonor for the phosphorylation of various response regulators in vitro and, in the absence of the cognate sensor kinases, acetyl phosphate can also modulate the activities of several response regulators in vivo. Here, we demonstrate increased in vivo half-lives of sigma(S) and the RpoS742::LacZ hybrid protein (also a substrate for RssB-dependent proteolysis) in acetyl phosphate-free (pta-ackA) deletion mutants, even though no sensor kinase was eliminated. The in vivo data indicate that acetyl phosphate acts through the response regulator, RssB. In vitro, efficient phosphotransfer from radiolabelled acetyl phosphate to the Asp-58 residue of RssB (the expected site of

Author

Sandrine Bouché; Eberhard Klauck; Daniela Fischer; Magnus Lucassen; Kirsten Jung; Regine Hengge‐Aronis

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)