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Can I read “On the Collander equation”: Protein partitioning in polymer/polymer aqueous two-phase systems on EtoBox?
“On the Collander equation”: Protein partitioning in polymer/polymer aqueous two-phase systems by Pedro P. Madeira; José A. Teixeira; Eugénia A. Macedo; Larissa M. Mikheeva; Boris Y. Zaslavsky is a Chemistry article available to read on EtoBox.
What is “On the Collander equation”: Protein partitioning in polymer/polymer aqueous two-phase systems about?
Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation: ln K i = a io ln K o + b io , where K i and K o are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients a io and b io are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison.
Who reads “On the Collander equation”: Protein partitioning in polymer/polymer aqueous two-phase systems?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Pedro P. Madeira; José A. Teixeira; Eugénia A. Macedo; Larissa M. Mikheeva; Boris Y. Zaslavsky
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2008
- Language
- EN
- Field
- Chemistry (Physical Sciences)