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Can I read Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides on EtoBox?
Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides by Shu-ichi Nakano; Toshimasa Kirihata; Satoshi Fujii; Hiroshi Sakai; Masayasu Kuwahara; Hiroaki Sawai; Naoki Sugimoto is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides about?
A self-complementary nucleotide sequence can form both a unimolecular hairpin and a bimolecular duplex. In this study, the secondary structures of the self-complementary DNA and RNA oligonucleotides with different sequences and lengths were investigated under various solution conditions by gel electrophoresis, circular dichroism (CD) and electron paramagnetic resonance (EPR) spectroscopy and a ultraviolet (UV) melting analysis. The DNA sequences tended to adopt a hairpin conformation at low cation concentrations, but a bimolecular duplex was preferentially formed at an elevated cationic strength. On the other hand, fully matched RNA sequences adopted a bimolecular duplex regardless of the cation concentration. The thermal melting experiments indicated a greater change in the melting temperature of the bimolecular duplexes (by ∼20°C) than that of the hairpin (by ∼10°C) by increasing the NaCl concentration from 10 mM to 1 M. Hairpin formations were also observed for the palindrome DNA sequences derived from __Escherichia coli__, but association of the complementary palindrome sequences was observed when spermine, one of the major cationic molecules in a cell, existed at the physiolog
Who reads Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Shu-ichi Nakano; Toshimasa Kirihata; Satoshi Fujii; Hiroshi Sakai; Masayasu Kuwahara; Hiroaki Sawai; Naoki Sugimoto
- Publisher
- Oxford University Press
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)