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Study on the Three-dimensional Proliferation of Rabbit Articular Cartilage-derived Chondrocytes on Polyhydroxyalkanoate Scaffolds by Ying Deng; Kai Zhao; Xiong-fei Zhang; Ping Hu; Guo-Qiang Chen is a Engineering article available to read on EtoBox.

What is Study on the Three-dimensional Proliferation of Rabbit Articular Cartilage-derived Chondrocytes on Polyhydroxyalkanoate Scaffolds about?

Polymer scaffold systems consisting of poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx)/polyhydroxybutyrate (PHB) (PHBHHx/PHB) were investigated for possible application as a matrix for the three-dimensional growth of chondrocyte culture. Blend polymers of PHBHHx/PHB were fabricated into three-dimensional porous scaffolds by the salt-leaching method. Chondrocytes isolated from rabbit articular cartilage (RAC) were seeded on the scaffolds and incubated over 28 days, with change of the culture medium every 4 days. PHB scaffold was taken as a control. Methylthiazol tetrazolium (MTT) (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltertra-zolium bromide) assay was used to quantitatively examine the proliferation of chondrocytes. Results showed that chondrocytes proliferated better on the PHBHHx/PHB scaffolds than on PHB one. The maximal cell densities were all observed after 7 days of incubation. As for the blend polymers, cells grew better on scaffolds consisting of PHBHHx/PHB in ratios of 2:1 and 1:2 than they did on PHBHHx/PHB of 1:1. Scanning electron microscopy (SEM) also showed that large quantities of chondrocytes grew initially on the surface of the scaffold. After 7 days, they furt

Who reads Study on the Three-dimensional Proliferation of Rabbit Articular Cartilage-derived Chondrocytes on Polyhydroxyalkanoate Scaffolds?

Author

Ying Deng; Kai Zhao; Xiong-fei Zhang; Ping Hu; Guo-Qiang Chen

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

2002

Language

EN

Field

Engineering (Physical Sciences)