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Structural studies of the allelic wheat glutenin subunits 1Bx7 and 1Bx20 by matrix-assisted laser desorption/ionization mass spectrometry and high-performance liquid chromatography/electrospray ionization mass spectrometry by Vincenzo Cunsolo; Salvatore Foti; Rosaria Saletti; Simon Gilbert; Arthur S. Tatham; Peter R. Shewry is a Chemistry article available to read on EtoBox.

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## Abstract Structural studies of the high molecular mass (HMM) glutenin subunits 1Bx7 (from cvs Hereward and Galatea) and 1Bx20 (from cv. Bidi17) of bread wheat were conducted using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) and reversed‐phase high‐performance liquid chromatography/electrospray ionization mass spectrometry (RP‐HPLC/ESI‐MS). For all three proteins, MALDI‐TOFMS analysis showed that the isolated fractions contained a second component with a mass about 650 Da lower than the major component. The testing and correction of the gene‐derived amino acid sequences of the three proteins were performed by direct MALDI‐TOFMS analysis of their tryptic peptide mixture. Analysis of the digest was performed by recording several MALDI mass spectra of the mixture at low, medium and high mass ranges, optimizing the matrix and the acquisition parameters for each mass range. Complementary data were obtained by RP‐HPLC/ESI‐MS analysis of the tryptic digest. This resulted in coverage of about 98% of the sequences. In contrast to the gene‐derived data, the results obtained demonstrate the insertion of the sequence QPGQGQ between Trp^716^ and

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Author

Vincenzo Cunsolo; Salvatore Foti; Rosaria Saletti; Simon Gilbert; Arthur S. Tatham; Peter R. Shewry

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

2004

Language

EN

Field

Chemistry (Physical Sciences)