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Determination of Amphetamine-related Compounds by High-performance Liquid Chromatography with Chemiluminescence and Fluorescence Detections by Kazuichi Hayakawa; Kimie Hasegawa; Noriko Imaizumi; Osborne S. Wong; Motoichi Miyazaki is a Chemistry article available to read on EtoBox.
What is Determination of Amphetamine-related Compounds by High-performance Liquid Chromatography with Chemiluminescence and Fluorescence Detections about?
High-performance liquid chromatography with chemiluminescence detection has been established for the determination of trace levels of amphetamine-related compounds (APs) after fluorigenic derivatization. Bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide in acetonitrile was used as a post-column chemilumigenic reagent. As derivatization reagents, dansyl chloride (Dns-Cl), 4-fluoro-7-nitrobenzoxadiazole (NBD-F) and naphtalene-2,3\_dicarbaldehyde (NDA) were compared. Dns-Cl was the most suitable of the three for the simultaneous determination of both primary and secondary amino APs. The on-column detection limits for Dns-derivatives were 3 . 10-i' -4 -lo-i5 mol. NDA gave the most sensitive derivatives (cyanobenzV]isoindole, CBI derivatives) with only primary amino APs. The detection limits for CBI derivatives were as low as 2 . lo-l6 mol. The present method was applied to the determination of methamphetamine in human urine. Only diethyl ether extraction was necessary as a clean-up treatment before Dns derivatization, because diethyl ether extracted methamphetamine quantitatively from urine at strong alkaline pH and the extract showed few interfering peaks around the retention t
Who reads Determination of Amphetamine-related Compounds by High-performance Liquid Chromatography with Chemiluminescence and Fluorescence Detections?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Kazuichi Hayakawa; Kimie Hasegawa; Noriko Imaizumi; Osborne S. Wong; Motoichi Miyazaki
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1991
- Language
- EN
- Field
- Chemistry (Physical Sciences)