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Ethanol potentiates the function of the human dopamine transporter expressed in Xenopus oocytes by R. Dayne Mayfield; Rajani Maiya; Denise Keller; Nancy R. Zahniser is a Neuroscience article available to read on EtoBox.

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Ethanol alters a variety of properties of brain dopaminergic neurons including firing rate, synthesis, release, and metabolism. Recent studies suggest that ethanol's action on central dopamine systems may also involve modulation of dopamine transporter (DAT) activity. The human DAT was expressed in __Xenopus__ oocytes to examine directly the effects of ethanol on transporter function. [^3^H]Dopamine (100 nm) accumulation into DAT‐expressing oocytes increased significantly in response to ethanol (10 min; 10–100 mm). In two‐electrode voltage‐clamp experiments, DAT‐mediated currents were also enhanced significantly by ethanol (10–100 mm). The magnitude of the ethanol‐induced potentiation of DAT function depended on ethanol exposure time and substrate concentration. Cell surface DAT binding ([^3^H]WIN 35,428; 4 nm) also increased as a function of ethanol exposure time. Thus, the increase in dopamine uptake was associated with a parallel increase in the number of DAT molecules expressed at the cell surface. These experiments demonstrate that DAT‐mediated substrate translocation and substrate‐associated ionic conductances are sensitive to intoxicating concentrations of ethanol and sugges

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Author

R. Dayne Mayfield; Rajani Maiya; Denise Keller; Nancy R. Zahniser

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2001

Language

EN

Field

Neuroscience (Life Sciences)