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Enzyme-Mediated Ligation Methods by Timo Nuijens, Marcel Schmidt is a nonfiction available to read on EtoBox.
What is Enzyme-Mediated Ligation Methods about?
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, __Enzyme-Mediated Ligation Methods__ is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answ
Who reads Enzyme-Mediated Ligation Methods?
It is typically read by self-directed learners exploring a subject in depth.
Common subject areas: history, science, philosophy, social sciences.
- Author
- Timo Nuijens, Marcel Schmidt
- Publisher
- Springer New York : Imprint : Humana
- Published
- 2019
- Language
- EN
- ISBN
- 9781493995462
- Category
- nonfiction
- Subjects
- Science, Technology, Chemistry
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