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What is Intestinal Organoid-Microbe Cocultures about?

This protocol provides detailed methods for coculturing human intestinal organoids with microbes, particularly focusing on the small intestine and colon exposed to individual bacterial species. Microinjection directly into the lumen and periphery of polarized 3D organoids is discussed to model the natural epithelium-microbe orientation in the gut. Considerations are also given for assessing the coculture over time through various microscopy techniques and omics approaches to study the impact of bacteria on

Author

Marcelo Yudi Icimoto

Language

EN