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Comparison of the crystal structures of genetically engineered human manganese superoxide dismutase and manganese superoxide dismutase from thermus thermophilus : Differences in dimer–dimer interaction by Ulrike G. Wagner; Felix Frolow; Joel L. Sussman; Katherine A. Pattridge; Martha L. Ludwig; William C. Stallings; Moshe M. Werber; Christian Oefner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The three‐dimensional X‐ray structure of a recombinant human mitochondrial manganese superoxide dismutase (MnSOD) (chain length 198 residues) was determined by the method of molecular replacement using the related structure of MnSOD from __Thermus thermophilus__ as a search model. This tetrameric human MnSOD crystallizes in space group P2~1~2~1~2 with a dimer in the asymmetric unit (Wagner, U.G., Werber, M.M., Beck, Y., Hartman, J.R., Frolow, F., & Sussman, J.L., 1989, __J. Mol. Biol. 206__, 787–788). Refinement of the protein structure (3, 148 atoms with Mn and no solvents), with restraints maintaining noncrystallographic symmetry, converged at an __R__‐factor of 0.207 using all data from 8.0 to 3.2 Å resolution and group thermal parameters. The monomer–monomer interactions typical of bacterial Fe‐ and Mn‐containing SODs are retained in the human enzyme, but the dimer–dimer interactions that form the tetramer are very different from those found in the structure of MnSOD from __T. thermophilus__. In human MnSOD one of the dimers is rotated by 84° relative to its equivalent in the thermophile enzyme. As a result the monomers are arranged in an approximately tetrahedral a

Who reads Comparison of the crystal structures of genetically engineered human manganese superoxide dismutase and manganese superoxide dismutase from thermus thermophilus : Differences in dimer–dimer interaction?

Author

Ulrike G. Wagner; Felix Frolow; Joel L. Sussman; Katherine A. Pattridge; Martha L. Ludwig; William C. Stallings; Moshe M. Werber; Christian Oefner

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)