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The document discusses a method for determining hydroxocobalamin and cyanocobalamin in biological media using first derivative spectrophotometry, which allows for direct measurement without extraction. This method is particularly useful in cases of cyanide poisoning, where rapid assessment of these compounds is critical for effective treatment. The study demonstrates that the technique is fast, reliable, and has a detection limit of approximately 5 µM for both compounds.

Author

pagasablo

Language

EN