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Design, Synthesis and Properties of Synthetic Chlorophyll Proteins by Harald K. Rau; Heike Snigula; Andreas Struck; Bruno Robert; Hugo Scheer; Wolfgang Haehnel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Design, Synthesis and Properties of Synthetic Chlorophyll Proteins about?

A chemoselective method is described for coupling chlorophyll derivatives with an aldehyde group to synthetic peptides or proteins modified with an aminoxyacetyl group at the ε‐amino group of a lysine residue. Three template‐assembled antiparallel four‐helix bundles were synthesized for the ligation of one or two chlorophylls. This was achieved by coupling unprotected peptides to cysteine residues of a cyclic decapeptide by thioether formation. The amphiphilic helices were designed to form a hydrophobic pocket for the chlorophyll derivatives. Chlorophyll derivatives Zn‐methylpheophorbide __b__ and Zn‐methyl‐pyropheophorbide __d__ were used. The aldehyde group of these chlorophyll derivatives was ligated to the modified lysine group to form an oxime bond. The peptide–chlorophyll conjugates were characterized by electrospray mass spectrometry, analytical HPLC, and UV/visible spectroscopy. Two four‐helix bundle chlorophyll conjugates were further characterized by size‐exclusion chromatography, circular dichroism, and resonance Raman spectroscopy.

Who reads Design, Synthesis and Properties of Synthetic Chlorophyll Proteins?

Author

Harald K. Rau; Heike Snigula; Andreas Struck; Bruno Robert; Hugo Scheer; Wolfgang Haehnel

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)