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Interaction of CD44 variant isoforms with hyaluronic acid and the cytoskeleton in human prostate cancer cells by Catherine F. Welsh; Dan Zhu; Lilly Y. W. Bourguignon is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Interaction of CD44 variant isoforms with hyaluronic acid and the cytoskeleton in human prostate cancer cells about?

CD44 is a glycosylated adhesion molecule which may undergo alternative splicing of 10 possible exons to generate variant isoforms. A number of CD44 variant isoforms expressed by tumor cells have been correlated with metastatic and proliferative behavior. In this study, we have characterized CD44 isoform expression on three prostate cancer cell lines: ALVA-31, PPC-1, and LNCaP. Using reverse transcriptase-polymerase chain reaction, we have found that ALVA-31 and PPC-1 cells express multiple CD44 isoforms, including CD44s (standard form), CD44E (epithelial form), and an exon 14-containing form. In addition, two smaller forms have been detected: one using an alternative donor splice site within exon 5, and a novel form omitting exon 5 entirely. The CD44 isoforms expressed by ALVA-31 and PPC-1 cells appear to be preferentially located on the cell surface. By contrast, LNCaP cells do not express any of the CD44 forms at the RNA or protein level. Both PPC-1 and ALVA-31 cells display tumorigenesis and invasiveness in nude mice, whereas LNCap cells exhibit a less malignant phenotype, suggesting a correlation between CD44 variant (CD44v) expression and aggressive prostate tumor behavior. Fu

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Author

Catherine F. Welsh; Dan Zhu; Lilly Y. W. Bourguignon

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)