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Flow Cytometric Study of in Vitro Neutrophil Activation by Biomaterials by M. B. Gorbet; E. L. Yeo; M. V. Sefton is a Medicine article available to read on EtoBox.
What is Flow Cytometric Study of in Vitro Neutrophil Activation by Biomaterials about?
Neutrophil activation for adherent and nonadherent cells, as measured by flow cytometry, was not strongly dependent on material surface chemistry. We had hypothesized that material-induced neutrophil activation was an important parameter associated with material failure. All materials tested [cellophane, an acrylonitrile copolymer (AN69), Pellethane, nylon, polyethylene terephthalate, low density polyethylene, and polydimethylsiloxane] activated isolated human neutrophils, which were resuspended in plasma or serum, to similar extents based on L-selectin shedding, CD11b upregulation, and stimulation of the oxidative burst after 30-min exposure. Inhibition of complement activation by sCR1 unexpectedly had little effect if any on nonadherent neutrophils. However, neutrophil adhesion, but not the level of activation of the adherent cells, was strongly dependent on complement activation. Pretreatment with albumin did not inhibit adhesion or reduce neu-trophil activation, but plasma pretreatment resulted in increased activation for nonadherent and adherent cells. More adhesion and a higher level of activation of adherent cells was observed following pretreatment with fibrinogen, a ligand
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- Author
- M. B. Gorbet; E. L. Yeo; M. V. Sefton
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Heterocorporation; Wiley (ISSN 0021-9304)
- Published
- 1999
- Language
- EN
- Field
- Medicine (Health Sciences)