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Report of a european collaborative exercise comparing DNA typing results using a single locus VNTR probe by P.M. Schneider; R. Fimmers; S. Woodroffe; D.J. Werrett; W. Bär; B. Brinkmann; B. Eriksen; S. Jones; A.D. Kloosterman; B. Mevåg; V.L. Pascali; C. Rittner; H. Schmitter; J.A. Thomson; P. Gill is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Report of a european collaborative exercise comparing DNA typing results using a single locus VNTR probe about?

A collaborative exercise was carried out in 1989 among 12 European forensic laboratories using the single locus VNTR probe pYNH24, the restriction enzyme HinfI, the same set of human genomic DNA samples, and a standardized DNA size marker. The objectives of the exercise were: (1) to study the degree of variation within and between laboratories, (2) to obtain information on requirements for technical standardization allowing the exchange of typing results and (3) to compare different approaches for the identification of allelic DNA fragments of unknown size. Each laboratory carried out up to 10 independent typing experiments using the same DNA samples. The results were analysed independently by two laboratories using three different methods. The results of the exercise demonstrate the correlation of typing that can be achieved within and between laboratories under conditions of minimal standardization.

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Author

P.M. Schneider; R. Fimmers; S. Woodroffe; D.J. Werrett; W. Bär; B. Brinkmann; B. Eriksen; S. Jones; A.D. Kloosterman; B. Mevåg; V.L. Pascali; C. Rittner; H. Schmitter; J.A. Thomson; P. Gill

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)