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Ion Chromatograph with Immobilized Alkaline Phosphatase Reactor for the Determination of Adenosine Phosphates by Sanae Ikeda; Hideki Morino; Junko Motonaka; Yuji Mishima is a Chemistry article available to read on EtoBox.

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An ion chromatograph equipped with an immobilized enzyme reactor was developed for the determination of adenosine phosphates. The enzyme reactor was prepared by immobilization of alkaline phosphatase onto activated glass beads. The proposed equipment made it possible to determine adenosine phosphates rapidly as phosphate ion. Adenosine monophosphate (AMP, 510 À5 ±210 À4 M), adenosine diphosphate (ADP, 110 À5 ±110 À4 M) and adenosine triphosphate (ATP, 110 À5 ± 110 À4 M) could be determined precisely with a relative error of less than 3.6%, in the presence of L-lactic acid, creatine, myoglobin, albumin, chloride, nitrate, phosphate, and sulfate ions.

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Author

Sanae Ikeda; Hideki Morino; Junko Motonaka; Yuji Mishima

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

1999

Language

EN

Field

Chemistry (Physical Sciences)