Can I read Characterization and Modeling of Monolithic Stationary Phases: Application to Preparative Chromatography on EtoBox?

Characterization and Modeling of Monolithic Stationary Phases: Application to Preparative Chromatography by Sanchayita Ghose; Steven M. Cramer is a Chemistry article available to read on EtoBox.

What is Characterization and Modeling of Monolithic Stationary Phases: Application to Preparative Chromatography about?

A methodology for characterizing and modeling preparative separations on monolithic ion-exchange stationary phases is presented. A dimensionless group analysis was carried out to determine the relative importance of mass transfer and kinetic resistances on this stationary phase. In contrast to conventional beaded morphologies, the continuous bed stationary phase was found to possess enhanced mass transport properties resulting in kinetic resistance as the dominant non-ideality. Accordingly, a reaction-dispersive steric-mass action formalism was successfully utilized for simulating preparative displacement chromatography on this resin. Since kinetics were found to be important on this column morphology, mobile phase salt concentration was found to be an important variable during displacement chromatography on this stationary phase. An increase in the mobile phase salt concentration was found to significantly improve the displacement separation of a model protein mixture. The formalism presented in this paper provides a better understanding of preparative chromatography in monolithic resin systems and a means of simulating separations on this class of chromatographic stationary phase

Who reads Characterization and Modeling of Monolithic Stationary Phases: Application to Preparative Chromatography?

Author

Sanchayita Ghose; Steven M. Cramer

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2001

Language

EN

Field

Chemistry (Physical Sciences)