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Caveolin Expression and Localization in Human Keratinocytes Suggest a Role in Lamellar Granule Biogenesis by G.N. Sando; H. Zhu; J.M. Weis; J.T. Richman; K.C. Madison; P.W. Wertz is a Medicine article available to read on EtoBox.

What is Caveolin Expression and Localization in Human Keratinocytes Suggest a Role in Lamellar Granule Biogenesis about?

Lamellar granules are sphingolipid-enriched organelles, probably intimately related to the tubulo-vesicular elements of the trans-Golgi network, that deliver the precursors of stratum corneum barrier lipids to the extracellular compartment. Caveolins are cholesterol-binding scaffolding proteins that facilitate the assembly of cholesterol- and sphingolipid-enriched membrane domains known as caveolae. Similarities in the composition of lamellar granules and caveolae suggest that caveolins could be involved in lamellar granule assembly, trafficking, and/or function. In order to explore this relationship, we have examined the expression of caveolins in epidermis, keratinocyte cultures, and an isolated lamellar granule fraction using immunolabeling, immunoblotting, and northern blotting. Several antibodies show immunolocalization of caveolin-1 in the basal layer of human epidermis, with a decline in the suprabasal layers and a reemergence of expression at the stratum granulosum/stratum corneum junction. Two of three caveolin-2 antibodies show little basal staining, but strong signal throughout the rest of the epidermis, whereas a third shows a pattern like caveolin-1. An antibody agains

Who reads Caveolin Expression and Localization in Human Keratinocytes Suggest a Role in Lamellar Granule Biogenesis?

Author

G.N. Sando; H. Zhu; J.M. Weis; J.T. Richman; K.C. Madison; P.W. Wertz

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; Springer Science and Business Media LLC; Wiley (ISSN 0022-202X)

Published

2003

Language

EN

Field

Medicine (Health Sciences)