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Genome-wide identification, gene cloning, subcellular location and expression analysis of SPL gene family in P. granatum L by Bianbian Li; Yujie Zhao; Sha Wang; Xinhui Zhang; Yongwei Wang; Yu Shen; Zhaohe Yuan is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Genome-wide identification, gene cloning, subcellular location and expression analysis of SPL gene family in P. granatum L about?
## Abstract ## Backgrounds Pomegranate is an excellent tree species with nutritional, medicinal, ornamental and ecological values. Studies have confirmed that SPL factors play an important role in floral transition and flower development. ## Results Used bioinformatics methods, 15 SPL (SQUAMOSA promoter-binding protein-like) genes were identified and analyzed from the ‘Taishanhong’ pomegranate (P. granatum L.) genome. Phylogenetic analysis showed that PgSPLs were divided into six subfamilies (G1 ~ G6). PgSPL promoter sequences contained multiple cis-acting elements associated with abiotic stress or hormonal response. Based on the transcriptome data, expression profiles of different tissues and different developmental stages showed that PgSPL genes had distinct temporal and spatial expression characteristics. The expression analysis of miR156 in small RNA sequencing results showed that miR156 negatively regulated the expression of target genes. qRT-PCR analysis showed that the expression levels of PgSPL2, PgSPL3, PgSPL6, PgSPL11 and PgSPL14 in leaves were significantly higher than those in buds and stems (p < 0.05). The expression levels of PgSPL5, PgSPL12 and PgSPL13 in flower buds
Who reads Genome-wide identification, gene cloning, subcellular location and expression analysis of SPL gene family in P. granatum L?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Bianbian Li; Yujie Zhao; Sha Wang; Xinhui Zhang; Yongwei Wang; Yu Shen; Zhaohe Yuan
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)