Opening book details…
Can I read The Recent-Transmission of Mycobacterium tuberculosis Strains among Iranian and Afghan Relapse Cases: a DNA-fingerprinting using RFLP and spoligotyping on EtoBox?
The Recent-Transmission of Mycobacterium tuberculosis Strains among Iranian and Afghan Relapse Cases: a DNA-fingerprinting using RFLP and spoligotyping by Parissa-Farnia; Mohammad Reza Masjedi; Mohammad Varahram; Mehdi Mirsaeidi; Mojtaba Ahmadi; Mehdi Khazampour; Payam Tabarsi; Parvaneh Baghei; Mojtaba Marjane; Muslam Bahadori; Abolhasan Zia Zarifi; Ali Akbar Velayati is a Medicine article available to read on EtoBox.
What is The Recent-Transmission of Mycobacterium tuberculosis Strains among Iranian and Afghan Relapse Cases: a DNA-fingerprinting using RFLP and spoligotyping about?
## Background Relapse of tuberculosis (TB) may develop as the result of reactivation of the endogenous primary infection, or as a result of a exogenous reinfection. This survey evaluated the rate of reactivation versus recent transmission among Iranian and Afghan relapse cases. ## Methods The sputum specimens were digested, examined microscopically for acid-fast bacilli, and inoculated into Löwenstein-Jensen slants by standard procedures. Thereafter, the susceptibility and identification tests were performed on culture positive specimens. Subsequently, the strains that were identified as __Mycobacterium tuberculosis__ (258 isolates) were subjected to IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping. Additional patient's information was collected for further epidemiological analysis. Patients whose isolates had identical genotyping patterns were considered a cluster with recent transmission episode. ## Results Out of 258 available isolates, 72(28%) had multi-drug resistant (MDR-TB) in ratio and 42 (16.2%) had other resistant. Notably, 38 of MDR-TB cases (52%) were isolated from Afghan patients. By IS6110-RFLP typing method, 65 patients (25%) were clustered in
Who reads The Recent-Transmission of Mycobacterium tuberculosis Strains among Iranian and Afghan Relapse Cases: a DNA-fingerprinting using RFLP and spoligotyping?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Parissa-Farnia; Mohammad Reza Masjedi; Mohammad Varahram; Mehdi Mirsaeidi; Mojtaba Ahmadi; Mehdi Khazampour; Payam Tabarsi; Parvaneh Baghei; Mojtaba Marjane; Muslam Bahadori; Abolhasan Zia Zarifi; Ali Akbar Velayati
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2334)
- Published
- 2008
- Language
- EN
- Field
- Medicine (Health Sciences)