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Can I read 5-Fluorouracil disrupts ovarian preantral follicles in young C57BL6J mice on EtoBox?
5-Fluorouracil disrupts ovarian preantral follicles in young C57BL6J mice by Juliana Z. Almeida; Laritza F. Lima; Luís A. Vieira; Carolina Maside; Anna C. A. Ferreira; Valdevane R. Araújo; Ana B. G. Duarte; Ramon S. Raposo; Sônia N. Báo; Cláudio C. Campello; Luiz F. S. Oliveira; Thayse P. da Costa; José Garcia Abreu; José R. Figueiredo; Reinaldo B. Oriá is a Medicine article available to read on EtoBox.
What is 5-Fluorouracil disrupts ovarian preantral follicles in young C57BL6J mice about?
## Purpose: 5-fluorouracil (5-fu), an anti-cancer drug, has been used for hepatoblastoma (hb) chemotherapy in children, who may have impaired ovarian follicle pool reserve with lasting effects to reproduction. therefore, this study aimed to investigate 5-fu effects on survival, growth, and morphology of ovarian preantral follicles from c57bl6j young mice. ## Methods: Experiments were carried-out both in vivo and in vitro. mice were treated with 5-fu injection (450 mg/kg i.p) or saline and sacrificed 3 days after to obtain ovaries for histology and molecular biology. ovaries for in vitro studies were obtained from unchallenged mice and cultured under basic culture medium (bcm) or bcm plus 5-fu (9.2, 46.1, 92.2 mm). preantral follicles were classified according to developmental stages, and as normal or degenerated. to assess cell viability, caspase-3 immunostaining was performed. transcriptional levels for apoptosis (bax, bcl2, p53, bax/bcl2) and wnt pathway genes (wnt2 and wnt4) were also analyzed. ultrastructural analyses were carried-out on non-cultured ovaries. in addition, β-catenin immunofluorescence was assessed in mouse ovaries. ## Results: The percentage of all-types normal
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- Author
- Juliana Z. Almeida; Laritza F. Lima; Luís A. Vieira; Carolina Maside; Anna C. A. Ferreira; Valdevane R. Araújo; Ana B. G. Duarte; Ramon S. Raposo; Sônia N. Báo; Cláudio C. Campello; Luiz F. S. Oliveira; Thayse P. da Costa; José Garcia Abreu; José R. Figueiredo; Reinaldo B. Oriá
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)