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In This Issue is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Whooooooo has the answer to the dreaded black spot disease ooooo? This is not the black spot of Stevenson's __Treasure Island__, a piece of paper that only condemned one person to death, but an infectious fungus that condemns forests of poplar trees to massive defoliation and reduced photosynthesis. The fungus, __Marssonina brunnea__ is well studied microscopically, but is molecularly poorly characterized. To address this lacuna, Cheng __et al.__ characterized the fungal peptides and proteins secreted by the fungus in a free‐living state. Since no DNA or protein sequences were published, this required __de novo__ peptide and protein sequencing (ESI‐MS/MS) after growth and isolation of secreted products from culture medium. Previous steps included 2‐DE, two rounds of an improved degenerate primer PCR (aka CODEHOP), 5′‐ and 3′‐ RACE cloning of mRNAs, and RT‐PCR. The presence of signal sequences on mRNA and proteins confirmed a secreted role for many of the proteins. pp. 2406–2417 ## Double your pleasure, double your fun Double your protein IDs to make sure it's the one. (hmm, scans a bit rough). Platelets make an interesting proteome target because when mature, they ar

Who reads In This Issue?

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)