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Development of a High-throughput Enzyme-linked Immunosorbent Assay for the Routine Detection of the Carcinogen Acrylamide in Food, via Rapid Derivatisation Pre-analysis by Andrew Preston; Terence Fodey; Christopher Elliott is a Chemistry article available to read on EtoBox.

What is Development of a High-throughput Enzyme-linked Immunosorbent Assay for the Routine Detection of the Carcinogen Acrylamide in Food, via Rapid Derivatisation Pre-analysis about?

The spontaneous formation of the neurotoxic carcinogen acrylamide in a wide range of cooked foods has recently been discovered. These foods include bread and other bakery products, crisps, chips, breakfast cereals, and coffee. To date, the diminutive size of acrylamide (71.08 Da) has prevented the development of screening immunoassays for this chemical. In this study, a polyclonal antibody capable of binding the carcinogen was produced by the synthesis of an immunogen comprising acrylamide derivatised with 3-mercaptobenzoic acid (3-MBA), and its conjugation to the carrier protein bovine thyroglobulin. Antiserum from the immunised rabbit was harvested and fully characterised. It displayed no binding affinity for acrylamide or 3-MBA but had a high affinity for 3-MBAderivitised acrylamide. The antisera produced was utilised in the development of an ELISA based detection system for acrylamide. Spiked water samples were assayed for acrylamide content using a previously published extraction method validated for coffee, crispbread, potato, milk chocolate and potato crisp matrices. Extracted acrylamide was then subjected to a rapid 1-h derivatisation with 3-MBA, pre-analysis. The ELISA was

Who reads Development of a High-throughput Enzyme-linked Immunosorbent Assay for the Routine Detection of the Carcinogen Acrylamide in Food, via Rapid Derivatisation Pre-analysis?

Author

Andrew Preston; Terence Fodey; Christopher Elliott

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)