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Kinetic characterization of phosphoenolpyruvate carboxylase extracted from whole-leaf and from guard-cell protoplasts of Vicia faba L. (C3 plant) with respect to tissue pre-illumination by Xue-Chen Wang; William H. Outlaw; Jean André De Bedout; Zhirong Du is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Whole leaves and guard-cell protoplasts of the C 3 plant Vicia faba L. (broad bean) were separately extracted following a period of illumination or following a period of darkness. Kinetic parameters of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31), V~a x and K~Ep.~g ~, were determined as a function of assay pH (7.0 or 8.1), the presence of 5 mM glucose-6-P~ e (Glc-6-P, an activator), and the presence of 5 mM malat%e e (an inhibitor). On the basis of these parameters, guard-cell PEPC was distinguished from that of whole leaf, indicating either that guard cells contain a unique isoenzyme of PEPC or a different complement of isoenzymes or-and less likely-that the obligatorily different methodologies for the leaf (intact organ) and the guard-cell (protoplast) enzymes altered them specifically. The values of Vma x were relatively unchanged, regardless of assay conditions or tissue pretreatment. The values obtained for whole-leaf PEPC Vr~x were restricted to a small range (52.4 q-5.9 (SD) to 64.4 + 4.8 (SD) p, mol -g flesh mass -~. h ~; the high value coincided with the presence of Glc-6-P, and the low value was obtained in the presence of malate. Guard-cell PEPC Vm~ was also restr

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Author

Xue-Chen Wang; William H. Outlaw; Jean André De Bedout; Zhirong Du

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0018-2214)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)