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On crystallization of water confined in liposomes and cryoprotective action of DMSO by Klbik, Ivan (author);Čechová, Katarína (author);Maťko, Igor (author);Lakota, Ján (author);Šauša, Ondrej (author) is a Materials Science article available to read on EtoBox.

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In this work, the phase behavior of cryoprotective mixtures based on dimethyl sulfoxide (DMSO) mixed with a lipid bilayer consisting of dimyristoyl phosphatidylcholine (DMPC) was studied. This system represented a model of a biological cell and its membrane. The aim of the work was to clarify the origin of the cryoprotective action of low-concentrated mixtures (1–10 vol%) DMSO in water, representing mixtures used in cryopreservation in cell therapy. The combination of experimental techniques of differential scanning calorimetry (DSC) and positron annihilation lifetime spectroscopy (PALS) allowed a study of crystallization behavior of water confined in liposomes imitating the intracellular environment. The ability of liposomes to show the fundamental aspects of water phase behavior seen during freezing of biological cells was proved. The presence of an amorphous freeze-concentrated phase of DMSO in the frozen state was confirmed and its possible crystallization into the DMSO trihydrate and ice during thawing was demonstrated. Correlation between the critical temperature range for the loss of cell viability during slow thawing and the temperatures of freeze-concentrated phase crystal

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Author

Klbik, Ivan (author);Čechová, Katarína (author);Maťko, Igor (author);Lakota, Ján (author);Šauša, Ondrej (author)

Publisher

Royal Society of Chemistry (RSC)

Published

2022

Language

EN

Field

Materials Science (Physical Sciences)